This responsibility put Peyton Randolph at odds with Patrick Henry, the Virginian most noted for opposition to the tax. At the end of the legislative session in 1765, Henry, a freshman, introduced seven resolutions against the act. Peyton Randolph, George Wythe, and others thought that Henry's resolutions added nothing to the colony's case and that their consideration was improper until the colony had a reply to its earlier protests.
In the final days of the session, after many opponents had left the city, Patrick Henry introduced his measures and made the famous speech in which he said “Caesar had his Brutus, Charles the First had his Cromwell, and George the Third . . .” prompting cries of treason from the remaining burgesses present. Peyton Randolph, though not yet Speaker, was presiding. When Speaker John Robinson resumed the chair the following day (May 30), Henry carried five of his resolves by a single ballot. A tie would have allowed Robinson to cast the deciding "nay." Jefferson, standing at the chamber door, said Peyton Randolph emerged saying, "By God, I would have given one hundred guineas for a single vote."
On May 24, 1774, Robert Carter Nicholas introduced a resolution drafted by Thomas Jefferson that read:
"This House, being deeply impressed with apprehension of the great dangers, to be derived to British America, from the hostile Invasion of the City of Boston, in our Sister Colony of Massachusetts bay, whose commerce and harbour are, on the first Day of June next, to be stopped by an Armed force, deem it highly necessary that the said first day of June be set apart, by the Members of this House, as a day of Fasting, Humiliation and Prayer, devoutly to implore the divine interposition for averting the heavy Calamity which threatens destruction to our Civil Rights, and the Evils of civil War; to give us one heart and one Mind to firmly oppose, by all just and proper means, every injury to American Rights; and that the Minds of his Majesty and his parliament, may be inspired from above with Wisdom, Moderation, and Justice, to remove from the loyal People of America, all cause of danger, from a continued pursuit of Measure, pregnant with their ruin."
The resolution was adopted.
Governor Dunmore summoned the house on May 26, 1774 and told Peyton Randolph: "Mr. Speaker and Gentlemen of the House of Burgesses, I have in my hand a paper published by order of your House, conceived in such terms as reflect highly upon His Majesty and the Parliament of Great Britain, which makes it necessary for me to dissolve you; and you are accordingly dissolved."
On May 27, 1774, a group of 89 burgesses gathered again at the Raleigh Tavern to form another “non-importation association,” and the following day the Committee of Correspondence proposed a Continental Congress. Twenty-five burgesses met at Peyton Randolph's house on May 30 and scheduled a state convention to be held on August 1 to consider a proposal from Boston for a ban on exports to England.
Peyton Randolph led the community to Bruton Parish Church on June 1 to pray for Boston, and soon he was organizing a Williamsburg drive to send provisions and cash for its relief.
The First Virginia Convention approved the export ban and elected as delegates to the Congress Peyton Randolph, Richard Henry Lee, George Washington, Patrick Henry, Richard Bland, Benjamin Harrison, and Edmund Pendleton.
Peyton Randolph deliberates Peyton Randolph in the